Plant Metabolic Engineering Lab

Cross section of a sugarcane leaf  stained with Nile Blue A and viewed with a compound fluorescent  microscope. PHB granules are seen a small yellow granules in the  various leaf cell plastids with the high densities in the bundle sheath cells. Photo by Dr. Lars Petrasovits.

Brumbley Lab Group Photo

Plant Metabolic Engineering Lab

The Plant Engineering lab has four platforms. 

1.      Gene Discovery. The gene discovery group is looking for novel genes to feed into the plant metabolic engineering program. Environmental screening of samples uses PCR, next generation sequencing and metabolomics. Focus is on native algal populations.

2.      Development of green foxtail millet (Setaria viridis) as a model system for metabolic engineering of high biomass C4 grasses. S. viridis, the progenitor for foxtail millet (Setaria italica), is ideal for this type of research. S. viridis (Accession A10.1) has the characteristics of an ideal model system: it is a NADP-ME type C4 grass with a small diploid genome (490 Mb) that is conserved with the major grass lineages, it is self-compatible, it has a rapid reproductive rate and its genome has just been fully sequenced by Joint Genome Institute (JGI). Foxtail millet is also easily transformed and has a very short life cycle (30 to 56 days) allowing for rapid growth and assessment of metabolism and introduced enzymes or pathways for metabolic engineering and plant architecture.

3.      Development of sorghum as a biofactory for production of biopolymers, biopolymer precursors, biofuels, and fiber. Although sorghum is more difficult to transform than sugarcane, it can be grown on marginal soils and across the temperate regions.

4.      Sugarcane as a biofactory for biopolymers, biofuels and fiber. Building upon a decade of research, sugarcane is still a key focus for our plant biofactory program. 

 

Interview with Dr. Stevens Brumbley by the University of North Texas